James Nachtwey grew up in Massachusetts and graduated from Dartmouth College, where he studied Art History and Political Science (1966-70). Images from the Vietnam War and the American Civil Rights movement had a powerful effect on him and were instrumental in his decision to become a photographer. He has worked aboard ships in the Merchant Marine, and while teaching himself photography, he was an apprentice news film editor and a truck driver.

In 1976 he started work as a newspaper photographer in New Mexico, and in 1980, he moved to New York to begin a career as a freelance magazine photographer. His first foreign assignment was to cover civil strife in Northern Ireland in 1981 during the IRA hunger strike. Since then, Nachtwey has devoted himself to documenting wars, conflicts and critical social issues. He has worked on extensive photographic essays in El Salvador, Nicaragua, Guatemala, Lebanon, the West Bank and Gaza, Israel, Indonesia, Thailand, India, Sri Lanka, Afghanistan, the Philippines, South Korea, Somalia, Sudan, Rwanda, South Africa, Russia, Bosnia, Chechnya, Kosovo, Romania, Brazil, Iraq, and the United States.

Nachtwey has been a contract photographer with Time Magazine since 1984. In 2001, he became one of the founding members of the photo agency, VII. He was associated with Black Star from 1980 - 1985 and was a member of Magnum from 1986 until 2001. He has had solo exhibitions at the International Center of Photography in New York, the Bibliotheque Nationale de France in Paris, the Palazzo Esposizione in Rome, the Museum of Photographic Arts in San Diego, Culturgest in Lisbon, El Circulo de Bellas Artes in Madrid, Fahey/Klein Gallery in Los Angeles, the Massachusetts College of Art in Boston, the Canon Gallery and the Nieuwe Kerk in Amsterdam, the Carolinum in Prague,and the Hasselblad Center in Sweden, among others.